MDCK II cells expression occludin-mEmerald were cultured on a custom made compression device. Confocal z-stacks were taken before compression and afteer compression for 4 h in 30 min intervals. Zeiss LSM 780 confocal micrtoscope with C-Apochromat 63x/1.20 W Korr M27 objective was used.Image size 1024x1024 pixels, pixel size 131.8 nmZ-step 120 nmPxel dwell time 6.363636363636364e-007sChannel 1 DPSS 561 nmChannel 2 T-PMT

2022